Characterization of the protoplasmic constituents of bacillus megaterium.
نویسنده
چکیده
It has been shown (Weibull, 1953) that it is possible to isolate protoplasts from Bacillus megaterium by lysozyme treatment of cells suspended in 0.1 to 0.2 M sucrose. When protoplasts are resuspended in phosphate buffer (pH 7), rapid lysis occurs, and the protoplasmic constituents are released. This procedure does not include any mechanical disintegration; in addition, only a small fraction of the lysozyme initially applied is present in the final lysate so that enzymatic or nonenzymatic interaction between lysozyme and the protoplasmic constituents is largely avoided. This paper describes the properties of the protoplasmic material so obtained.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 66 6 شماره
صفحات -
تاریخ انتشار 1953