Characterization of the protoplasmic constituents of bacillus megaterium.

نویسنده

  • C WEIBULL
چکیده

It has been shown (Weibull, 1953) that it is possible to isolate protoplasts from Bacillus megaterium by lysozyme treatment of cells suspended in 0.1 to 0.2 M sucrose. When protoplasts are resuspended in phosphate buffer (pH 7), rapid lysis occurs, and the protoplasmic constituents are released. This procedure does not include any mechanical disintegration; in addition, only a small fraction of the lysozyme initially applied is present in the final lysate so that enzymatic or nonenzymatic interaction between lysozyme and the protoplasmic constituents is largely avoided. This paper describes the properties of the protoplasmic material so obtained.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and molecular characterization of bacillus megaterium isolated from different agroclimatic zones of karnataka and its effect on seed germination and plant growth of Sesamum indicum

Bacillus megaterium have been advocated as effective and economical bio inoculant to use in the integrated nutrient and pest control system. Bacillus megaterium from soil of different Agro climatic zones of Karnataka were isolated and identified. Molecular characterization of the Bacillus megaterium isolates was done using RAPD technique. The study of effect of Bacillus megaterium on seed germi...

متن کامل

Comparative proteomics analysis of a novel g-radiation-resistant bacterium wild-type Bacillus megaterium strain WHO DQ973298 recovering from 5 KGy g-irradiation

In order to examine radiation-induced proteins in an extremely radio-resistant bacterium, it became possibleto perform comparative proteomic analysis on radio-resistance Bacillus megaterium WHO as a wildtypestrain for the first time. Variation in cellular proteins profiles of the Bacillus megaterium WHO after 5KGy γ-irradiation were analyzed by two-dimensional poly acryl amide...

متن کامل

Genome-Scale Metabolic Network Models of Bacillus Species Suggest that Model Improvement is Necessary for Biotechnological Applications

Background: A genome-scale metabolic network model (GEM) is a mathematical representation of an organism’s metabolism. Today, GEMs are popular tools for computationally simulating the biotechnological processes and for predicting biochemical properties of (engineered) strains.Objectives: In the present study, we have evaluated the predictive power of two ...

متن کامل

Rhizobacteria for reduced fertilizer inputs in wheat (Triticum aestivum spp. vulgare) and barley (Hordeum vulgare) on Aridisols in Turkey

The present study assessed the effect of seed inoculation with single or multiple plant growth-promoting rhizobacterial (PGPR) strains on yield in spring wheat (Triticum aestivum spp. vulgare var. Kırık) and barley (Hordeum vulgare cv. Tokak) plants in both greenhouse and field conditions during the years 2007 and 2008. The treatments of wheat and barley plants during the first year include...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 66 6  شماره 

صفحات  -

تاریخ انتشار 1953